Jurkat-Human TIGIT (Luc)-Reporter Cell
| Catalog Number |
CBCL-0266 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
This Jurkat reporter cell line expresses the NFAT response element driving luciferase expression and the full-length human TIGIT receptor (Gene ID: 201633). It can be used to evaluate the potency of TIGIT blockade. When co-cultured with target cells expressing human CD155, the TIGIT/CD155 interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the TIGIT/CD155 interaction with anti-TIGIT or anti-CD155 antibodies releases the inhibitory signal, resulting in TCR activation and NFAT-mediated luminescence.
Application
Screen for anti-human TIGIT or anti-human CD155 antibody.
Background
TIGIT is an immune checkpoint receptor expressed on T cells and natural killer cells. It inhibits immune cell activation and plays a role in immune tolerance and tumor immune evasion.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
Jurkat-Human TIGIT (Luc)-Reporter Cell |
| Protein Family |
T cell immunoreceptor with Ig and ITIM domains family |
| Gene |
TIGIT |
| NCBI Gene ID |
201633 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) + Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
