Jurkat-NFAT (Luc)-Reporter Cell
| Catalog Number |
CBCL-0253 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The NFAT (Luc) Jurkat Reporter Cell is designed with the NFAT response element to drive luciferase expression. This cell line can be modified to express a chimeric antigen receptor (CAR) for developing a CAR-J-based activity screening system. Intracellular signals induced by anti-TCR/CD3 can be inhibited by transfected immune checkpoints binding to their corresponding ligands.
Application
Transfection host for some immune checkpoint concerning the NFAT signaling pathway
Background
NFAT (Nuclear Factor of Activated T Cells) is a transcription factor that regulates gene expression in response to calcium signaling in T cells and other immune cells.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
Jurkat-NFAT (Luc)-Reporter Cell |
| Protein Family |
Nuclear factor of activated T-cells family |
| Gene |
NFAT |
| NCBI Gene ID |
NFATC2/4773 |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin (5 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
