Jurkat-NFAT-Luc2-ACEII-CD3zeta-Reporter Cell Line
| Catalog Number |
CBCL-0283 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
This stable Jurkat cell line expresses exogenous human ACEII, CD3zeta, and luciferase under the control of the NFAT signaling pathway, enabling studies of immune receptor signaling and NFAT-dependent gene expression.
Application
Drug screening and biological assays
Background
ACE2 (Angiotensin-converting enzyme 2) is a key enzyme in the renin-angiotensin system (RAS) and serves as the receptor for the SARS-CoV-2 virus, which causes COVID-19. ACE2 is widely expressed in various tissues, including the lungs, heart, kidneys, and intestines, and plays a crucial role in regulating blood pressure and fluid balance. CD3-zeta (CD3Z) is a component of the T cell receptor (TCR) complex and is essential for TCR signaling and T cell activation. Mutations in the CD3Z gene can lead to immunodeficiency disorders characterized by recurrent infections and impaired T cell function.
Product Information
| Category |
Luciferase Reporter Cell Lines |
| Cell Line Name |
Jurkat-NFAT-Luc2-ACEII-CD3zeta-Reporter Cell Line |
| Protein Family |
Angiotensin converting enzyme family; T cell surface glycoprotein family |
| Gene |
ACEII; CD3-zeta |
| Species |
Human |
| Host Cell Line |
Jurkat |
| Cell Type |
Human T-cell leukemia |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin |
| Recommended Medium |
RPMI1640+10%FBS+300µg/mL Hygromycin B+0.75µg/mL Puromycin |
| Freeze Medium |
70% RPMI1640+20% FBS+10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
