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Jurkat-NFAT-Luc2-BTLA-CD3-zeta-Stable Cell Line

Catalog Number CBCL-0273
Price Online Inquiry
Unit Quantity 1 x 10^6 cells / 1.0 ml

Introduction

This stable Jurkat cell line expresses exogenous human BTLA and CD3 zeta, along with luciferase under the control of the NFAT signaling pathway, facilitating studies of immune receptor signaling and NFAT-dependent gene expression.

Application

Drug screening and biological assays

Background

Product Information

Category Luciferase Reporter Cell Lines
Cell Line Name Jurkat-NFAT-Luc2-BTLA-CD3-zeta-Stable Cell Line
Protein Family B and T lymphocyte attenuator family; T cell surface glycoprotein family
Gene BTLA; CD3-zeta
Species Human
Host Cell Line Jurkat
Cell Type Human T-cell leukemia
Morphology Lymphoblastoid
Growth Properties Suspension
Selection Marker Puromycin
Recommended Medium RPMI1640+10%FBS+300µg/mL Hygromycin B+0.75µg/mL Puromycin
Freeze Medium 70% RPMI1640+20% FBS+10% DMSO
Storage Method Frozen in liquid nitrogen.
Stability Validated for at least 10 passages
Mycoplasma Negative
Sterility Testing Negative
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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