Jurkat-NFAT-Luc2-CTLA4-CD3-zeta-Stable Cell Line
| Catalog Number | CBCL-0279 |
| Price | Online Inquiry |
| Unit Quantity | 1 x 10^6 cells / 1.0 ml |
Introduction
This stable Jurkat cell line expresses exogenous human CTLA4 and CD3 zeta, along with luciferase under the control of the NFAT signaling pathway, enabling studies of immune receptor signaling and NFAT-dependent gene expression.
Application
Drug screening and biological assays
Background
Product Information
| Category | Luciferase Reporter Cell Lines |
| Cell Line Name | Jurkat-NFAT-Luc2-CTLA4-CD3-zeta-Stable Cell Line |
| Protein Family | Cytotoxic T-lymphocyte-associated protein 4 family; T cell surface glycoprotein family |
| Gene | CTLA4; CD3-zeta |
| Species | Human |
| Host Cell Line | Jurkat |
| Cell Type | Human T-cell leukemia |
| Morphology | Lymphoblastoid |
| Growth Properties | Suspension |
| Selection Marker | Puromycin |
| Recommended Medium | RPMI1640+10%FBS+300µg/mL Hygromycin B+0.75µg/mL Puromycin |
| Freeze Medium | 70% RPMI1640+20% FBS+10% DMSO |
| Storage Method | Frozen in liquid nitrogen. |
| Stability | Validated for at least 10 passages |
| Mycoplasma | Negative |
| Sterility Testing | Negative |
| Shipping | Shipping in dry ice |
| Biosafety Level | BSL-1 |
| Caution | For Research Use Only |
For research use only. Not for any other purpose.