KRAS-G12C-H95Q Stable Ba/F3 Cell Line
| Catalog Number |
CBCL-74 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable Ba/F3 clone expressing exogenous KRAS bearing G12C and H95Q double mutation.
Application
Drug screening and biological assays
Background
The KRAS gene provides instructions for making a protein called K-Ras that is part of a signaling pathway known as the RAS/MAPK pathway. The overactivation due to mutation can lead to continuous cell proliferation, and eventually develop into cancer.
Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
Product Information
| Species |
Mouse |
| Host Cell Line |
Ba/F3 cell line |
| Cell Type |
Pro-B cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Suspension |
| Pharmaceutical Target |
Kinases |
| Recommended Medium |
RPMI 1640 + 10% FBS |
| Selection Marker |
Puromycin |
| Morphology |
Mostly round |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:10. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 20 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
