MCF7-Tet-off-GFP-Stable Cell Line
| Catalog Number |
CBCL-0356 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
This MCF7 cell line has been engineered with a tetracycline (Tet)-off system to express green fluorescent protein (GFP). GFP expression can be regulated by the presence or absence of tetracycline, allowing for controlled studies of gene expression and cellular dynamics.
Application
Drug screening and biological assays
Background
Tet-off is a system used to control gene expression in cells, where the presence of tetracycline or its analogs represses transcription of a target gene.
Product Information
| Category |
Fluorescence Labeled Cell Lines |
| Cell Line Name |
MCF7-Tet-off-GFP-Stable Cell Line |
| Protein Family |
Tetracycline-controlled transcriptional activation family |
| Gene |
Tet-off |
| Species |
Human |
| Host Cell Line |
MCF7 |
| Cell Type |
Human breast cancer |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Selection Marker |
Hygromycin B |
| Recommended Medium |
RPMI1640+10%FBS+1mg/mL G418+200µg/mL Hygromycin B |
| Freeze Medium |
70% RPMI1640+20% FBS+10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
