| Catalog Number | CBCL-0362 |
| Price | Online Inquiry |
| Unit Quantity | 1 x 10^6 cells / 1.0 ml |
This stable MDA-MB-231 cell line has a PDL1 gene knockout and expresses exogenous human PD1v mutant, CD81, and GFP, facilitating studies of immune checkpoint interactions and visualization of PD1v localization.
Drug screening and biological assays
| Category | Fluorescence Labeled Cell Lines |
| Cell Line Name | MDA-MB-231-PDL1-KO-PD1v-CD81-GFP-Stable Cell Line |
| Protein Family | Programmed cell death ligand 1 family; Programmed cell death protein 1 family; Tetraspanin family |
| Gene | PDL1; PD1v; CD81 |
| Species | Human |
| Host Cell Line | MDA-MB-231 |
| Cell Type | Human breast cancer |
| Morphology | Epithelial |
| Growth Properties | Adherent |
| Selection Marker | Puromycin |
| Recommended Medium | RPMI1640+10%FBS+1µg/mL Puromycin+100µg/mL Hygromycin B |
| Freeze Medium | 70% RPMI1640+20% FBS+10% DMSO |
| Storage Method | Frozen in liquid nitrogen. |
| Stability | Validated for at least 10 passages |
| Mycoplasma | Negative |
| Sterility Testing | Negative |
| Shipping | Shipping in dry ice |
| Biosafety Level | BSL-1 |
| Caution | For Research Use Only |