ME-180-HRE Luciferase Reporter-Stable Cell Line
| Catalog Number |
CBCL-0736 |
| Price |
Online Inquiry |
| Unit Quantity |
2 x 10^6 cells / 1.0 ml |
Introduction
The ME-180-HRE Luciferase Reporter-Stable Cell Line is designed to monitor the activity of hypoxia-inducible factors (HIFs) through a firefly luciferase reporter driven by multiple copies of the hypoxia response element (HRE). This cell line has been validated in response to hypoxia-mimetics like cobalt chloride and Deferoxamine.
Application
Drug screening and biological assays
Background
HRE (Hypoxia - Response Element) is a cis - acting DNA element that is recognized and bound by hypoxia - inducible factors (HIFs). It is present in the promoter regions of hypoxia - responsive genes and regulates gene expression in response to low oxygen levels, playing important roles in processes such as angiogenesis, tumor growth, and adaptation to hypoxia.
Product Information
| Category |
Cell Signaling Cell Lines |
| Cell Line Name |
ME-180-HRE Luciferase Reporter-Stable Cell Line |
| Protein Family |
Hypoxia response element family |
| Gene |
HRE |
| Species |
Human |
| Host Cell Line |
ME-180 |
| Cell Type |
Human cervical carcinoma |
| Morphology |
Epithelial |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
90% FBS + 10% DMSO |
| Storage Method |
Liquid Nitrogen |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
