Mouse CD40L Stable NIH/3T3 Cell Line

Catalog Number

CBCL-527

Price

Online Inquiry

Unit Quantity

1x10⁶ cells / 1.0 ml

Introduction

NIH/3T3 cell line stable expressing exogenous mouse CD40L sequence

Application

Drug screening and biological assays

Background

D154, also named as CD40L ligand, is protein of TNF superfamily, mainly expressed on activated T cell, functioned as CD40 ligand, plays critical in immune response, such as B cell maturation, macrophage activation.

Product Information

Species	Mouse
Host Cell Line	NIH/3T3
Cell Type	Mouse embryonic fibroblast cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	90% DMEM + 10% FBS +0.5 μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Monolayer
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 24 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.