Mouse CD40L Stable NIH/3T3 Cell Line
| Catalog Number | CBCL-527 |
| Price | Online Inquiry |
| Unit Quantity | 1x106 cells / 1.0 ml |
Introduction
NIH/3T3 cell line stable expressing exogenous mouse CD40L sequence
Application
Drug screening and biological assays
Background
D154, also named as CD40L ligand, is protein of TNF superfamily, mainly expressed on activated T cell, functioned as CD40 ligand, plays critical in immune response, such as B cell maturation, macrophage activation.
Product Information
| Category | Immune Molecules Cell Lines |
| Cell Line Name | Mouse CD40L Stable NIH/3T3 Cell Line |
| Pharmaceutical Target | Immune Molecules |
| Species | Mouse |
| Host Cell Line | NIH/3T3 |
| Cell Type | Mouse embryonic fibroblast cells |
| Morphology | Monolayer |
| Growth Properties | Adherent |
| Selection Marker | Puromycin |
| Seeding Density | Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio | Recommended a ratio of 1:4-1:5. |
| Doubling Time | Approximately 24 hours |
| Recommended Medium | 90% DMEM + 10% FBS +0.5 μg/ml Puromycin |
| Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Stability | Stable in culture over 10 passages |
| QC | WB |
| Shipping | Shipping in dry ice |
| Biosafety Level | BSL-1 |
| Caution | For Research Use Only |
For research use only. Not for any other purpose.