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Mouse FAP Stable NIH/3T3 Cell Line

Catalog Number CBCL-372
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

NIH/3T3 cell line stable expressing exogenous mouse FAP sequence

Application

Drug screening and biological assays

Background

Fibroblast activation protein alpha (FAP) also named as prolyl endopeptidase FAP is a membrane-bound gelatinase, the expression of which is very low human tissue under physiological condition, and is high inactivated stromal fibroblasts of many human carcinoma.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name Mouse FAP Stable NIH/3T3 Cell Line
Pharmaceutical Target Immune Molecules
Species Mouse
Host Cell Line NIH/3T3
Cell Type Mouse embryonic fibroblast cells
Morphology Monolayer
Growth Properties Adherent
Selection Marker Puromycin and hygromycin B
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Doubling Time Approximately 24 hours
Recommended Medium 90% DMEM + 10% FBS + 1 μg/ml puromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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