Mouse FAP Stable NIH/3T3 Cell Line
| Catalog Number |
CBCL-372 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
NIH/3T3 cell line stable expressing exogenous mouse FAP sequence
Application
Drug screening and biological assays
Background
Fibroblast activation protein alpha (FAP) also named as prolyl endopeptidase FAP is a membrane-bound gelatinase, the expression of which is very low human tissue under physiological condition, and is high inactivated stromal fibroblasts of many human carcinoma.
Product Information
| Species |
Mouse |
| Host Cell Line |
NIH/3T3 |
| Cell Type |
Mouse embryonic fibroblast cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
90% DMEM + 10% FBS + 1 μg/ml puromycin |
| Selection Marker |
Puromycin and hygromycin B |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 24 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
