NF-kb Luciferase Reporter Stable HT1080 Cell Line

Catalog Number	CBCL-360
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HT1080 cell line stable expressing exogenous luciferase gene under the control of NFkb responsive element

Application

Drug screening and biological assays

Background

NFkB pathway plays an important role in controlling many biological processes including transcription of DNA, cytokine production, cell survival, immune responses to infection, developmental processes, synaptic plasticity, memory and cancer development. In response to the various stimuli, NFkB pathway is activated and NFKB protein complex translocate from cytoplasm to nucleus, regulates a wide spectrum of gene expression after binds to its response element on the promoter region of downstream gene.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	NF-kb Luciferase Reporter Stable HT1080 Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	HT1080
Cell Type	Fibrosarcoma, Fibroblasts
Morphology	Fibroblast
Growth Properties	Adherent
Selection Marker	Hygromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 48 hours
Recommended Medium	RPMI1640 + 10% FBS + 150 μg/ml Hygromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.