Catalog Number | CBCL-360 |
Price | Online Inquiry |
Unit Quantity | 1x106 cells / 1.0 ml |
HT1080 cell line stable expressing exogenous luciferase gene under the control of NFkb responsive element
Drug screening and biological assays
NFkB pathway plays an important role in controlling many biological processes including transcription of DNA, cytokine production, cell survival, immune responses to infection, developmental processes, synaptic plasticity, memory and cancer development. In response to the various stimuli, NFkB pathway is activated and NFKB protein complex translocate from cytoplasm to nucleus, regulates a wide spectrum of gene expression after binds to its response element on the promoter region of downstream gene.
Category | Immune Molecules Cell Lines |
Cell Line Name | NF-kb Luciferase Reporter Stable HT1080 Cell Line |
Pharmaceutical Target | Immune Molecules |
Species | Human |
Host Cell Line | HT1080 |
Cell Type | Fibrosarcoma, Fibroblasts |
Morphology | Fibroblast |
Growth Properties | Adherent |
Selection Marker | Hygromycin |
Seeding Density | Thaw entire contents into an appropriate T25 flask. |
Subculture Ratio | Recommended a ratio of 1:4-1:5. |
Doubling Time | Approximately 48 hours |
Recommended Medium | RPMI1640 + 10% FBS + 150 μg/ml Hygromycin |
Storage Method | Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
Stability | Stable in culture over 10 passages |
QC | WB |
Shipping | Shipping in dry ice |
Biosafety Level | BSL-1 |
Caution | For Research Use Only |