NIH3T3-Gli Luciferase Reporter-Stable Cell Line (Hedgehog Pathway)
| Catalog Number |
CBCL-0724 |
| Price |
Online Inquiry |
| Unit Quantity |
2 x 10^6 cells / 1.0 ml |
Introduction
The NIH3T3-Gli Luciferase Reporter-Stable Cell Line (Hedgehog Pathway) is designed for monitoring the activity of the hedgehog signaling pathway. It contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway.
Application
Drug screening and biological assays
Background
GLI1 (GLI Family Zinc Finger 1) is a transcription factor that is a key effector of the Hedgehog signaling pathway. It regulates the expression of target genes involved in cell proliferation, differentiation, and development, and its dysregulation is associated with various cancers and developmental disorders.
Product Information
| Category |
Cell Signaling Cell Lines |
| Cell Line Name |
NIH3T3-Gli Luciferase Reporter-Stable Cell Line (Hedgehog Pathway) |
| Protein Family |
Hedgehog signaling pathway family |
| Gene |
Gli |
| NCBI Gene ID |
2735 |
| Species |
Human |
| Host Cell Line |
NIH/3T3 |
| Cell Type |
Mouse embryonic fibroblast |
| Morphology |
Fibroblast-like |
| Growth Properties |
Adherent |
| Recommended Medium |
DMEM medium + 10% FBS |
| Freeze Medium |
90% FBS + 10% DMSO |
| Storage Method |
Liquid Nitrogen |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
