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NRAS-G12S Stable Ba/F3 Cell Line

Catalog Number CBCL-23
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable Ba/F3 clone expressing exogenous NRAS bearing G12S amino acid mutation.

Application

Drug screening and biological assays

Background

The NRAS proto-oncogene is a member of the Ras gene family, NRAS protein can bind GDP/GTP and possess intrinsic GTPase activity. The NRAS gene provides instructions for making a protein called N-Ras that is involved primarily in regulating cell division. Mutations in this gene lead to abnormal persistent activation of downstream genes such as Raf kinases, leading to tumorigenesis. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.

Product Information

Species Mouse
Host Cell Line Ba/F3 cell line
Cell Type Pro-B cells
Stability Stable in culture over 10 passages
Growth Properties Suspension
Pharmaceutical Target Kinases
Recommended Medium RPMI 1640 + 10% FBS
Selection Marker Puromycin
Morphology Mostly round
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:10.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 20 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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