OS8-hB7H4 Stable 293T Cell Line

Catalog Number	CBCL-518
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HEK293T cell line stable expressing membrane OKT3 scFV and human B7H4 gene

Application

Drug screening and biological assays

Background

B7H4, also named as B7x and B7S1, is transmembrane glycosylated protein belonging to B7 family of immunomodulatory proteins. B7H4 is expressed on the surface of many immune cells, including activated lymphocytes, macrophages, monocytes, dendritic cells, it can dampen T cells response and induces cell cycle arrest of T cells.

Product Information

Category	Immune Molecules Cell Lines
Cell Line Name	OS8-hB7H4 Stable 293T Cell Line
Pharmaceutical Target	Immune Molecules
Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Morphology	Monolayer
Growth Properties	Adherent
Selection Marker	Puromycin & Hygromycin
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Doubling Time	Approximately 30 hours
Recommended Medium	DMEM + 10% FBS + 0.5 μg/ml Puromycin + 50 μg/ml Hygromycin
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability	Stable in culture over 10 passages
QC	WB
Shipping	Shipping in dry ice
Biosafety Level	BSL-1
Caution	For Research Use Only

For research use only. Not for any other purpose.