OS8-hB7H4 Stable 293T Cell Line

Catalog Number	CBCL-518
Price	Online Inquiry
Unit Quantity	1x10⁶ cells / 1.0 ml

Introduction

HEK293T cell line stable expressing membrane OKT3 scFV and human B7H4 gene

Application

Drug screening and biological assays

Background

B7H4, also named as B7x and B7S1, is transmembrane glycosylated protein belonging to B7 family of immunomodulatory proteins. B7H4 is expressed on the surface of many immune cells, including activated lymphocytes, macrophages, monocytes, dendritic cells, it can dampen T cells response and induces cell cycle arrest of T cells.

Product Information

Species	Human
Host Cell Line	293T
Cell Type	Human embryonic kidney cells
Stability	Stable in culture over 10 passages
Growth Properties	Adherent
Pharmaceutical Target	Immune Molecules
Recommended Medium	DMEM + 10% FBS + 0.5 μg/ml Puromycin + 50 μg/ml Hygromycin
Selection Marker	Puromycin & Hygromycin
Morphology	Monolayer
Seeding Density	Thaw entire contents into an appropriate T25 flask.
Subculture Ratio	Recommended a ratio of 1:4-1:5.
Storage Method	Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time	Approximately 30 hours
QC	WB
Biosafety Level	BSL-1
Shipping	Shipping in dry ice
Caution	For Research Use Only

For research use only. Not for any other purpose.