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OS8-hB7H4 Stable 293T Cell Line

Catalog Number CBCL-518
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing membrane OKT3 scFV and human B7H4 gene

Application

Drug screening and biological assays

Background

B7H4, also named as B7x and B7S1, is transmembrane glycosylated protein belonging to B7 family of immunomodulatory proteins. B7H4 is expressed on the surface of many immune cells, including activated lymphocytes, macrophages, monocytes, dendritic cells, it can dampen T cells response and induces cell cycle arrest of T cells.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name OS8-hB7H4 Stable 293T Cell Line
Pharmaceutical Target Immune Molecules
Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Morphology Monolayer
Growth Properties Adherent
Selection Marker Puromycin & Hygromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Doubling Time Approximately 30 hours
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin + 50 μg/ml Hygromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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