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OS8-Human-PDL1 Stable 293T Cell Line

Catalog Number CBCL-529
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous membrane OKT3 scFV and human PD-L1 sequence

Application

Drug screening and biological assays

Background

PD-L1, also called human programed cell death ligand 1, is a transmembrane protein that play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease, virus infection and cancer. PD-L1 binds to its receptor PD-1 on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition. Upregulation of PD-L1 can allow cancer cell to evade the host immune system. 293T-OS8 stable cell was used as an artificial antigen presenting cells (APCs), OS8 could function as a membrane anchored T cell engager that directly activates TCRinT cell-based assay.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name OS8-Human-PDL1 Stable 293T Cell Line
Pharmaceutical Target Immune Molecules
Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Morphology Monolayer
Growth Properties Adherent
Selection Marker Hygromycin & Puromycin
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Doubling Time Approximately 30 hours
Recommended Medium DMEM + 10% FBS + 0.5 μg/ml Puromycin + 50 μg/ml Hygromycin
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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