OS8-Siglec15 Stable CHOK1 Cell Line
| Catalog Number |
CBCL-564 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
CHOK1 cell line stable expressing membrane OKT3 scFV and human Siglec15 gene
Application
Drug screening and biological assays
Background
293T-OS8 stable cell was used as an artificial antigen presenting cells (APCs), OS8 could function as a membrane anchored T cell engager that directly activates TCR in T cell-based assay.
Siglec-15 is a transmembrane glycoprotein, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family, and is found primarily on the surface of immune cells. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
Product Information
| Species |
Mouse |
| Host Cell Line |
CHOK1 |
| Cell Type |
Chinese hamster ovary cell line |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
F12K + 10% FBS + 5 μg/ml Puromycin + 100 μg/ml Hygromycin |
| Selection Marker |
Puromycin & Hygromycin |
| Morphology |
Epithelial |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 24 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
