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OS8 Stable 293T Cell Line

Catalog Number CBCL-519
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

HEK293T cell line stable expressing exogenous membrane OKT3 scFV

Application

Drug screening and biological assays

Background

293T-OS8 stable cell was used as an artificial antigen presenting cells (APCs), OS8 could function as a membrane anchored T cell engager that directly activates TCRinT cell-based assay.

Product Information

Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Stability Stable in culture over 10 passages
Growth Properties Adherent
Pharmaceutical Target Immune Molecules
Recommended Medium DMEM + 10% FBS + 150 μg/ml Hygromycin
Selection Marker Hygromycin
Morphology Monolayer
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling Time Approximately 30 hours
QC WB
Biosafety Level BSL-1
Shipping Shipping in dry ice
Caution For Research Use Only
For research use only. Not for any other purpose.
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