P95HER2 Stable T47D Cell Line
| Catalog Number |
CBCL-433 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable T47D cell clone expressing exogenous HER2 gene with extracellular domain deletion.
Application
Drug screening and biological assays
Background
HER2, also named ERBB2, is a cell-surface receptor tyrosine kinase, ERBB2 overexpression or overactivity have associated with a number of cancers, especially the breast cancer. The identification of ERBB2 as a driver gene has led to the development of anticancer therapeutics agents.
Product Information
| Species |
Human |
| Host Cell Line |
T47D cell line |
| Cell Type |
Mammary gland epithelial cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
RPMI 1640 + 10% FBS + 500 μg/ml G418 |
| Selection Marker |
Neomycin |
| Morphology |
Epithelial |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
