Raji-Human CD155-Stable Cell Line
| Catalog Number |
CBCL-0575 |
| Price |
Online Inquiry |
| Unit Quantity |
5 x 10^6 cells / 1.0 ml |
Introduction
The Raji-Human CD155-Stable Cell Line is engineered to express full-length human CD155 (Uniprot: P15151-1), used to mimic cancer target cells. When co-cultured with human TIGIT Reporter Cell, the TIGIT/CD155 interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the TIGIT/CD155 interaction by either anti-TIGIT or anti-CD155 antibodies releases the inhibitory signal and results in TCR activation and NFAT-mediated luminescence.
Application
Useful for cell-based CD155 binding assay
Background
CD155 (PVR, Poliovirus Receptor) is a transmembrane protein expressed on epithelial cells and tumor cells. It binds to DNAM-1 (activating) and TIGIT/CD96 (inhibitory) receptors on immune cells, modulating cytotoxicity and immune escape. Overexpression correlates with poor cancer prognosis.
Product Information
| Category |
Immune Checkpoint Cell Lines |
| Cell Line Name |
Raji-Human CD155-Stable Cell Line |
| Protein Family |
Nectin family |
| Gene |
CD155 |
| NCBI Gene ID |
5817 |
| Species |
Human |
| Host Cell Line |
Raji |
| Cell Type |
Human B-cell lymphoma |
| Morphology |
Lymphoblastoid |
| Growth Properties |
Suspension |
| Selection Marker |
Hygromycin B (20 μg/mL) |
| Recommended Medium |
RPMI-1640 + 10% FBS |
| Freeze Medium |
Serum-free cell cryopreservation medium |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
