SRE-Luc2-RET-GFRAL Stable 293T Cell Line
| Catalog Number |
CBCL-369 |
| Price |
Online Inquiry |
| Unit Quantity |
1x106 cells / 1.0 ml |
Introduction
Stable HEK293T cell line expressing exogenous luciferase gene under the control of SRE responsive element as well as exogenous RET and GFRAL protein.
Application
Drug screening and biological assays
Background
Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), is a divergent member of the transforming growth factor beta (TGF-beta) superfamily. GFRAL (GDNF receptor-alpha-like) is a functional receptor of GDF15, facilitating weight-loss functions of the protein through c-Ret downstream signaling.
Product Information
| Species |
Human |
| Host Cell Line |
293T |
| Cell Type |
Human embryonic kidney cells |
| Stability |
Stable in culture over 10 passages |
| Growth Properties |
Adherent |
| Pharmaceutical Target |
Immune Molecules |
| Recommended Medium |
RPMI1640 + 10% FBS + 1 μg/ml Puromycin + 150 μg/ml Hygromycin B |
| Selection Marker |
Puromycin and hygromycin B |
| Morphology |
Monolayer |
| Seeding Density |
Thaw entire contents into an appropriate T25 flask. |
| Subculture Ratio |
Recommended a ratio of 1:4-1:5. |
| Storage Method |
Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO |
| Doubling Time |
Approximately 30 hours |
| QC |
WB |
| Biosafety Level |
BSL-1 |
| Shipping |
Shipping in dry ice |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
