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SRE-Luc2-RET-GFRAL Stable 293T Cell Line

Catalog Number CBCL-369
Price Online Inquiry
Unit Quantity 1x106 cells / 1.0 ml

Introduction

Stable HEK293T cell line expressing exogenous luciferase gene under the control of SRE responsive element as well as exogenous RET and GFRAL protein.

Application

Drug screening and biological assays

Background

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), is a divergent member of the transforming growth factor beta (TGF-beta) superfamily. GFRAL (GDNF receptor-alpha-like) is a functional receptor of GDF15, facilitating weight-loss functions of the protein through c-Ret downstream signaling.

Product Information

Category Immune Molecules Cell Lines
Cell Line Name SRE-Luc2-RET-GFRAL Stable 293T Cell Line
Pharmaceutical Target Immune Molecules
Species Human
Host Cell Line 293T
Cell Type Human embryonic kidney cells
Morphology Monolayer
Growth Properties Adherent
Selection Marker Puromycin and hygromycin B
Seeding Density Thaw entire contents into an appropriate T25 flask.
Subculture Ratio Recommended a ratio of 1:4-1:5.
Doubling Time Approximately 30 hours
Recommended Medium RPMI1640 + 10% FBS + 1 μg/ml Puromycin + 150 μg/ml Hygromycin B
Storage Method Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Stability Stable in culture over 10 passages
QC WB
Shipping Shipping in dry ice
Biosafety Level BSL-1
Caution For Research Use Only
For research use only. Not for any other purpose.
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