THP1-IDH2-R140Q-GFP-Cell Line
| Catalog Number |
CBCL-0354 |
| Price |
Online Inquiry |
| Unit Quantity |
1 x 10^6 cells / 1.0 ml |
Introduction
This THP1 cell line has been modified to express the IDH2 R140Q mutant protein fused to green fluorescent protein (GFP), facilitating the study of this specific mutant's localization and dynamics.
Application
Drug screening and biological assays
Background
IDH2 R140Q is a point mutation in the IDH2 gene that leads to the production of an oncometabolite, 2-hydroxyglutarate, and is associated with certain cancers.
Product Information
| Category |
Fluorescence Labeled Cell Lines |
| Cell Line Name |
THP1-IDH2-R140Q-GFP-Cell Line |
| Protein Family |
Isocitrate dehydrogenase family |
| Gene |
IDH2 R140Q |
| NCBI Gene ID |
3418 |
| Species |
Human |
| Host Cell Line |
THP1 |
| Cell Type |
Human acute monocytic leukemia |
| Morphology |
Monocytic |
| Growth Properties |
Suspension |
| Selection Marker |
Puromycin |
| Recommended Medium |
RPMI1640+10%FBS+1µg/mL Puromycin |
| Freeze Medium |
70% RPMI1640+20% FBS+10% DMSO |
| Storage Method |
Frozen in liquid nitrogen. |
| Stability |
Validated for at least 10 passages |
| Mycoplasma |
Negative |
| Sterility Testing |
Negative |
| Shipping |
Shipping in dry ice |
| Biosafety Level |
BSL-1 |
| Caution |
For Research Use Only |
For research use only. Not for any other purpose.
